The Chemical PE Thread

The idea was to put BMP-4 into DMSO to avoid injection.

Mechanisms of Penile Erection and Basis for Pharmacolo​gical Treatment of Erectile Dysfunctio​n

Mechanisms of Penile Erection and Basis for Pharmacological Treatment of Erectile Dysfunction | Pharmacological Reviews

Wow more then you ever wanted to know… LOL

BMP-4

Check in my previous post about SHH\Sonic Hedgehog it hits on articles of BMP-4. Dont know if the molecule is small enough to flow through the skin via DMSO, It depends on the molecular weight and size of the molecule. Probably look up the molecular weight of BMP-4 and find out the limit of what DMSO can get through the skin.

Inuic

On PGE2 and analogs

PG-CL (Cloprostenol Sodium) (0.5 mg/Amp.):.
PGE1 (Prostaglandin E1) for injection (100mcg/vial):
PGF2a (Prostaglandin F2a) injection (50mg PGF2a per 10mL):

This is what I have seen available.

The two articles on these are awesome someone just previously posted!

Inuic

Thanks for your contributions, inuic.

But, ho-ho-hold on a minute, this is getting a tad too fast for men of my age ;)

You named quite a bunch of new APIs / methods which I’d rather sort out one after the other.

Here’s a little overview, to get some structure:

- Sonic hedgehog gene / protein (SHH)

Sonic hedgehog protein - Wikipedia
Inuic: studies about SHH

- Hydrogen Sulfide (H2S)

Hydrogen sulfide - Wikipedia
Inuic: studies about hydrogen sulphide and angiogenes​is

- Matrix Metallopeptidase 9 (MMP9)

MMP9 - Wikipedia

- Platelet Rich Plasma (PRP) (re-)injection ("priapus shot")

One interesting thing to note about MMP-9 is that like PRP which can be activated by Thrombin, can also activate MMP-9. Which kick starts some extra processes.

Controlled Delivery of Sonic Hedgehog Morphogen and Its Potential for Cardiac Repair - PMC

Sonic Hedgehog for Cardiac Repair? Smooth Muscle???

Hmm?

MMPs seem like a pretty valuable addition. Generally, from my understanding MMPs are essentially collagenases or gelatinases. If we identify the types of collagen we want to target, we can then pick the corresponding MMPs (there’s many, and each one targets different types of collagen), figure out the best method of application, and see if they have any benefit.

I haven’t read much on them, but I’m certainly curious about things like BMP-4 and SHH. I was under the impression that they were a part of a much bigger picture (as I’m merely an enthusiast and not a person well-versed in this matter), but there very well may be some merit in their use. Those studies are pretty interesting and I wish I had more time to dissect them further. They do seem like two compounds worth looking into, at the least.

Also, SometimesIGrow, most complex compounds won’t work with DMSO. It really can only carry things with a very low molecular weight through the skin. BMP-4 is definitely too big.

Molecular mass

1 Dalton [Da] = 1 gram per mol [g/mol]
1 kilo [k] = 1000

Insuline: 5.808 kDa

HRLN2: 2.7 kDa / 3.9 kDa (A chain / B chain)

IGF1: 7.649 kDa

BFGF: 18 kDa up to 25 kDa

VEGF: 19 kDa

PGE-1: 354 Da
PGE-2: 352 Da
PGF2a: 354 Da

TB4: 6.6 kDa

BMP-4: 34 kDa

Thrombine: 72 kDa

SHH: 45 kDa

MMP9: 88 kDa / 105 kDa / 125 kDa (proenzyme form / active form / complex)

Oxt: 1 kDa

DMSO: 78 Da

H2S: 34 Da

H2O: 18 Da

EDIT:

GHK-Cu: 340 Da

PABA: 137 Da

Colchicine: 399 Da

Pentoxifylline: 278 Da

Verapamil: 454 Da

Dalton (unit) - Wikipedia
Mole (unit) - Wikipedia

Doubleweener,

Nice listing of the masses…. Now the question is what is the threshold for what will get through the skin. If my memory serves correct that IGF-1 was just small enough to get into the skin via DMSO? Hence anything smaller would be a candidate for use with DMSO. Like isn’t HGH just to big for instance? What is the limit?

Inuic…

Also anyone ever used or hear of “hyaluronidase” I sourced this stuff and am going to add it to my protocol. This does the kinds of things DMSO does, get stuff deeper. I want to use this with my Relaxin-2.

“Hyaluronidase is a spreading or diffusing substance,which modifies the permeability of connective tissue through the hydrolysis of hyaluronic acid,a polysaccharide found in the intercellular ground substance of connective tissue, and of certain specialized tissues,such as the umbilical cord and vitreous humor.”

This may go good with MMP-9 also…

BTW does anyone know if Human Relaxin-2 stings when injected?

Inuic

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Hypersensitivity to hyaluronidase.order hyaluornidase online.

Not to be used to reduce the swelling of bites or stings or at sites where infection or malignancy is present.

Not to be used for anaesthetic procedures in cases of unexplained premature labour.

Not to be used for intravenous injections because the enzyme is rapidly inactivated.

Not to be used to enhance the absorption and dispersion of dopamine and/or alpha agonist drugs.

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Do not apply directly to the cornea.

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7.Interactions with other Medicines

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8.DOSAGE AND ADMINISTRATION

Hyaluronidase (hyaluronidase for injection) should be administered only as discussed below, since its effects relative to absorption and dispersion of other drugs are not produced when it is administered intravenously.

Hyaluronidase is to be reconstituted in the vial to a concentration of 1000 Units/mL of Sodium Chloride Injection, USP by adding 6.2 mL of solution to the vial. Prior to administration, the reconstituted solution should be further diluted to the desired concentration, commonly 150 Units/mL, see table below. The resulting solution should be used immediately after preparation.

A 1mL syringe and a 5-micron filter needle are supplied in the Hyaluronidase kit. Following reconstitution of Hyaluronidase, as described above, apply the 5-micron filter needle to the 1mL syringe. Draw the desired amount of Hyaluronidase into the syringe, and dilute according to the table below. Remove the filter needle and apply a needle appropriate for the intended injection.wholesale

Desired Concentration

Amount of hyaluronidase reconstituted solution

Additional Saline

(1000 Units/mL)

Chloride Injection

50 Units/mL

0.05 mL

0.95 mL

75 Units/mL

0.075 mL

0.925 mL

150 Units/mL

0.15 mL

0.85 mL

300 Units/mL

0.3 mL

0.7 mL

9.PRESENTATION AND STORAGE CONDITIONS Hyaluronidase 1500iu

Each ampoule contains 1,500 international units of Hyaluronidase for Injection BP (ovine) and sodium hydroxide. 1mL neutral glass ampoule containing a white or pale yellowish white, sterile, freeze-dried powder of the enzyme hyaluronidase for subcutaneous and intramuscular injection.order hyaluornidase 1500iu online.Protect from light. Store unopened vial in refrigerator at 2-8oC (35-46oF) . After reconstitution,store at controlled room temperature 20-25oC (68-77oF),and use within 6 hours.buy hyaluronidase 1500iu online.

Insulin-like growth factor-1 gene delivery may enhance the proliferation of human corpus cavernosal smooth muscle cells.

CONCLUSIONS:
IGF-1 gene transfer into HCCSMCs enhanced cellular proliferation, which was mediated by secretion of IGF-1. Our results suggest that IGF-1 gene therapy may be applied to corpus cavernosum regeneration.

Insulin-like growth factor-1 gene delivery may enhance the proliferation of human corpus cavernosal smooth muscle cells - PubMed

Love it

Inuic

Hey all,

Since the topic of transdermals came up - I was thinking it might be of some benefit to compile a list of substances that would fit in with ChemPE/PE in general. So far I’ve got:
Potaba: Softens Collagen
D-Penicillamine: Promotes elongation of collagen by inhibiting collagen cross-linkage
Colchicine: Ability to inhibit Collagen secretion from fibroblasts.
Pentoxifylline: Decreases Collagen, Fibronectin and Glycosaminoglycan production
Verapamil: “May be capable of increasing collagen breakdown”

Keep in mind that I’m unsure how accessible most of these chemicals are aside from Potaba/Paba, but compiling such a list may be of some assistance. I know the topic of topical PE aid(s) has come up often on here, but I think it fits well in this thread. Definitely interested in hearing about others anyone else may be aware of.

Thanks to a mod, I have updated my previous post to make a correction and add further APIs. Potaba and Paba should be very similar in molecular mass (needs to be checked). In line with the 500 Dalton rule, there are quite a few candidates that could be interesting for trans-dermal application. At least up to the point where the API reaches the tough and dense structures of the tunica. The question is however, whether aggressive collagen softeners cause damage to the skin before ever reaching the tunica. In most cases, only specific collagen types are attacked (by releasing its correspondent type of collagenase / proteinase), but what if one of the attacked collagen types is also a significant part of the outer dermis?

Agreed. It’s worth considering the well-being of the skin and also whether or not the compound in question can reach/properly interact with the tunica. The ones I’ve listed seem to be commonly used for other purposes with little side effects though.
Tunica interaction is definitely important to look into. For example, although Verapamil can allegedly break down collagen, but a study (Transdermal application of verapamil gel to the penile shaft fails to infiltrate the tunica albuginea - PubMed) found that "the gel does not infiltrate the tunica albuginea".

I guess in our case, there’s not too much science to be found backing up whether certain compounds interact with the tunica in the way that we want. I suppose trial and error would be necessary in this situation, really. Regardless, I’m curious if anyone has any other compounds of interest in regard to transdermal application.