Check in my previous post about SHH\Sonic Hedgehog it hits on articles of BMP-4. Dont know if the molecule is small enough to flow through the skin via DMSO, It depends on the molecular weight and size of the molecule. Probably look up the molecular weight of BMP-4 and find out the limit of what DMSO can get through the skin.
On PGE2 and analogs
PG-CL (Cloprostenol Sodium) (0.5 mg/Amp.):. PGE1 (Prostaglandin E1) for injection (100mcg/vial): PGF2a (Prostaglandin F2a) injection (50mg PGF2a per 10mL):
This is what I have seen available.
The two articles on these are awesome someone just previously posted!
Thanks for your contributions, inuic.
But, ho-ho-hold on a minute, this is getting a tad too fast for men of my age ;)
You named quite a bunch of new APIs / methods which I’d rather sort out one after the other.
Sonic Hedgehog for Cardiac Repair? Smooth Muscle???
MMPs seem like a pretty valuable addition. Generally, from my understanding MMPs are essentially collagenases or gelatinases. If we identify the types of collagen we want to target, we can then pick the corresponding MMPs (there’s many, and each one targets different types of collagen), figure out the best method of application, and see if they have any benefit.
I haven’t read much on them, but I’m certainly curious about things like BMP-4 and SHH. I was under the impression that they were a part of a much bigger picture (as I’m merely an enthusiast and not a person well-versed in this matter), but there very well may be some merit in their use. Those studies are pretty interesting and I wish I had more time to dissect them further. They do seem like two compounds worth looking into, at the least.
Also, SometimesIGrow, most complex compounds won’t work with DMSO. It really can only carry things with a very low molecular weight through the skin. BMP-4 is definitely too big.
1 Dalton [Da] = 1 gram per mol [g/mol] 1 kilo [k] = 1000
Insuline: 5.808 kDa
HRLN2: 2.7 kDa / 3.9 kDa (A chain / B chain)
IGF1: 7.649 kDa
BFGF: 18 kDa up to 25 kDa
VEGF: 19 kDa
PGE-1: 354 Da PGE-2: 352 Da PGF2a: 354 Da
TB4: 6.6 kDa
BMP-4: 34 kDa
Thrombine: 72 kDa
SHH: 45 kDa
MMP9: 88 kDa / 105 kDa / 125 kDa (proenzyme form / active form / complex)
The mass per mole of a substance is called its molar mass. Since the standard unit for expressing the mass of molecules or atoms (atomic mass unit or the dalton) is defined as 1/12 of the mass of a 12C atom. It follows that the molar mass of a substance. Measured in grams per mole. Is exactly equal to its mean molecular or atomic mass. Measured in unified atomic mass units or daltons; which is to say. To the substance’s mean molecular or relative atomic mass.
Nice listing of the masses…. Now the question is what is the threshold for what will get through the skin. If my memory serves correct that IGF-1 was just small enough to get into the skin via DMSO? Hence anything smaller would be a candidate for use with DMSO. Like isn’t HGH just to big for instance? What is the limit?
Also anyone ever used or hear of “hyaluronidase” I sourced this stuff and am going to add it to my protocol. This does the kinds of things DMSO does, get stuff deeper. I want to use this with my Relaxin-2.
“Hyaluronidase is a spreading or diffusing substance,which modifies the permeability of connective tissue through the hydrolysis of hyaluronic acid,a polysaccharide found in the intercellular ground substance of connective tissue, and of certain specialized tissues,such as the umbilical cord and vitreous humor.”
This may go good with MMP-9 also…
BTW does anyone know if Human Relaxin-2 stings when injected?
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Amount of hyaluronidase reconstituted solution
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Insulin-like growth factor-1 gene delivery may enhance the proliferation of human corpus cavernosal smooth muscle cells.
CONCLUSIONS: IGF-1 gene transfer into HCCSMCs enhanced cellular proliferation, which was mediated by secretion of IGF-1. Our results suggest that IGF-1 gene therapy may be applied to corpus cavernosum regeneration.
Since the topic of transdermals came up - I was thinking it might be of some benefit to compile a list of substances that would fit in with ChemPE/PE in general. So far I’ve got: Potaba: Softens Collagen D-Penicillamine: Promotes elongation of collagen by inhibiting collagen cross-linkage Colchicine: Ability to inhibit Collagen secretion from fibroblasts. Pentoxifylline: Decreases Collagen, Fibronectin and Glycosaminoglycan production Verapamil: “May be capable of increasing collagen breakdown”
Keep in mind that I’m unsure how accessible most of these chemicals are aside from Potaba/Paba, but compiling such a list may be of some assistance. I know the topic of topical PE aid(s) has come up often on here, but I think it fits well in this thread. Definitely interested in hearing about others anyone else may be aware of.
Thanks to a mod, I have updated my previous post to make a correction and add further APIs. Potaba and Paba should be very similar in molecular mass (needs to be checked). In line with the 500 Dalton rule, there are quite a few candidates that could be interesting for trans-dermal application. At least up to the point where the API reaches the tough and dense structures of the tunica. The question is however, whether aggressive collagen softeners cause damage to the skin before ever reaching the tunica. In most cases, only specific collagen types are attacked (by releasing its correspondent type of collagenase / proteinase), but what if one of the attacked collagen types is also a significant part of the outer dermis?
Last edited by Doubleweener : 12-19-2013 at .
Agreed. It’s worth considering the well-being of the skin and also whether or not the compound in question can reach/properly interact with the tunica. The ones I’ve listed seem to be commonly used for other purposes with little side effects though. Tunica interaction is definitely important to look into. For example, although Verapamil can allegedly break down collagen, but a study (http://www.ncbi.nlm.nih.gov/pubmed/12441945) found that “the gel does not infiltrate the tunica albuginea”.
I guess in our case, there’s not too much science to be found backing up whether certain compounds interact with the tunica in the way that we want. I suppose trial and error would be necessary in this situation, really. Regardless, I’m curious if anyone has any other compounds of interest in regard to transdermal application.